| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee
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| Autores: |
Fujii,Simone
Ono,Elisabete Yurie Sataque
Ribeiro,Ricardo Marcelo Reche
Assunção,Fernanda Garcia Algarte
Takabayashi,Cássia Reika
Oliveira,Tereza Cristina Rocha Moreira de
Itano,Eiko Nakagawa
Ueno,Yoshio
Kawamura,Osamu
Hirooka,Elisa Yoko
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| Data: |
2007-03-01
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| Ano: |
2007
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| Palavras-chave: |
Ochratoxin
Immunoassay
HPLC
Monoclonal antibody
Coffee
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| Resumo: |
An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA) detection in green, roasted and instant coffees was developed using anti-OTA monoclonal antibody. Immunological reagents prepared were OTA-BSA (4.76 mg/mL), anti-OTA.7 MAb (2x10³-fold dilution) and HRP-anti IgG (10³-fold dilution). The detection limit was 3.73 ng OTA/g and correlation coefficients (r) between this immunoassay and high performance liquid chromatography were 0.98 for green coffee, 0.98 for roasted and 0.86 for instant. OTA levels detected by ic-ELISA were higher than by HPLC, with ELISA/HPLC ratio of 0.66 - 1.46 (green coffee), 0.96 - 1.11 (roasted) and 0.93 - 1.82 (instant). ELISA recoveries for OTA added to coffee (5 - 70 ng/g) were 81.53 % for green coffee, 46.73 % for roasted and 64.35 % for instant, while recoveries by HPLC were 80.54 %, 45.91 % and 55.15 %, respectively. Matrices interferences were minimized by samples dilution before carrying out the ELISA assay. The results indicate that MAb-based ic-ELISA could be a simple, sensitive and specific screening tool for OTA detection, contributing to quality and safety of coffee products.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200020
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132007000200020
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.50 n.2 2007
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| Direitos: |
info:eu-repo/semantics/openAccess
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